🧬 RNA-seq Differential Expression

This skill performs differential expression on bulk RNA-seq or pseudo-bulk count matrices.

Core Capabilities

1. Input validation for count matrix and sample metadata
2. Pre-DE QC (library size, detected genes, low-count filtering)
3. PCA visualisation on normalized expression
4. Differential expression from formula + contrast
5. Volcano and MA plots
6. Markdown report with reproducibility files

Input Contract

• Count matrix (

  .csv

  or

  .tsv

  ): rows are genes, columns are samples, first column is gene identifier
• Metadata table (

  .csv

  or

  .tsv

  ): one row per sample, must include

  sample_id

• Formula: e.g.

  ~ condition

  or

  ~ batch + condition

• Contrast:

  factor,numerator,denominator

  (e.g.

  condition,treated,control

  )

Output Structure

rnaseq_de_report/
├── report.md
├── figures/
│   ├── pca.png
│   ├── volcano.png
│   └── ma_plot.png
├── tables/
│   ├── qc_summary.csv
│   ├── normalized_counts.csv
│   └── de_results.csv
└── reproducibility/
    ├── commands.sh
    ├── environment.yml
    └── checksums.sha256

Usage

python rnaseq_de.py \
  --counts counts.csv \
  --metadata metadata.csv \
  --formula "~ batch + condition" \
  --contrast "condition,treated,control" \
  --output report_dir

Safety

• Local-only processing
• Warn before overwriting existing output
• Report-level disclaimer required