name: bio-uniprot-access description: Access UniProt protein database for sequences, annotations, and functional information. Use when retrieving protein data, GO terms, domain annotations, or protein-protein interactions. tool_type: python primary_tool: requests measurable_outcome: Execute skill workflow successfully with valid output within 15 minutes. allowed-tools:

• read_file
• run_shell_command

UniProt Access

Query UniProt for protein sequences, functional annotations, and cross-references.

UniProt REST API

Fetch Single Entry

import requests

def fetch_uniprot(accession, format='fasta'):
    '''Fetch UniProt entry. Formats: fasta, json, txt, xml, gff'''
    url = f'https://rest.uniprot.org/uniprotkb/{accession}.{format}'
    response = requests.get(url)
    response.raise_for_status()
    return response.text

sequence = fetch_uniprot('P53_HUMAN', 'fasta')
entry_json = fetch_uniprot('P04637', 'json')

Search UniProt

def search_uniprot(query, format='json', size=25):
    '''Search UniProt with query syntax'''
    url = 'https://rest.uniprot.org/uniprotkb/search'
    params = {'query': query, 'format': format, 'size': size}
    response = requests.get(url, params=params)
    response.raise_for_status()
    return response.json() if format == 'json' else response.text

results = search_uniprot('gene:BRCA1 AND organism_id:9606')
for entry in results['results']:
    print(entry['primaryAccession'], entry['proteinDescription']['recommendedName']['fullName']['value'])

Query Syntax

gene:TP53

organism_id:9606

reviewed:true

length:[100 TO 500]

go:0006915

keyword:kinase

ec:2.7.1.1

database:pdb

Combine Queries

# Human kinases with structures
query = 'organism_id:9606 AND keyword:kinase AND database:pdb AND reviewed:true'
results = search_uniprot(query, size=100)

Batch Retrieval

Multiple Accessions

def batch_fetch(accessions, format='fasta'):
    '''Fetch multiple entries'''
    url = 'https://rest.uniprot.org/uniprotkb/accessions'
    params = {'accessions': ','.join(accessions), 'format': format}
    response = requests.get(url, params=params)
    return response.text

accessions = ['P04637', 'P53_HUMAN', 'Q9Y6K9']
sequences = batch_fetch(accessions)

Stream Large Results

def search_all(query, format='tsv', fields=None):
    '''Stream all results for large queries'''
    url = 'https://rest.uniprot.org/uniprotkb/stream'
    params = {'query': query, 'format': format}
    if fields:
        params['fields'] = ','.join(fields)
    response = requests.get(url, params=params, stream=True)
    return response.text

# Get all human proteins as TSV
all_human = search_all('organism_id:9606 AND reviewed:true',
                       fields=['accession', 'gene_names', 'protein_name'])

ID Mapping

Map Between Databases

import time

def map_ids(ids, from_db, to_db):
    '''Map IDs between databases'''
    url = 'https://rest.uniprot.org/idmapping/run'
    response = requests.post(url, data={'ids': ','.join(ids), 'from': from_db, 'to': to_db})
    job_id = response.json()['jobId']

    # Poll for results
    while True:
        status = requests.get(f'https://rest.uniprot.org/idmapping/status/{job_id}')
        if 'results' in status.json() or 'failedIds' in status.json():
            break
        time.sleep(1)

    results = requests.get(f'https://rest.uniprot.org/idmapping/results/{job_id}')
    return results.json()

# Ensembl gene IDs to UniProt
mapping = map_ids(['ENSG00000141510', 'ENSG00000171862'], 'Ensembl', 'UniProtKB')
for result in mapping['results']:
    print(result['from'], '->', result['to']['primaryAccession'])

Common Database Codes

UniProtKB

UniProtKB_AC-ID

Ensembl

RefSeq_Protein

PDB

GeneID

Gene_Name

Extract Specific Data

Parse JSON Entry

import json

entry = json.loads(fetch_uniprot('P04637', 'json'))

accession = entry['primaryAccession']
gene_name = entry['genes'][0]['geneName']['value']
protein_name = entry['proteinDescription']['recommendedName']['fullName']['value']
sequence = entry['sequence']['value']
length = entry['sequence']['length']

# GO terms
go_terms = [ref for ref in entry.get('uniProtKBCrossReferences', [])
            if ref['database'] == 'GO']

# Domains (InterPro)
domains = [ref for ref in entry.get('uniProtKBCrossReferences', [])
           if ref['database'] == 'InterPro']

# PDB structures
pdb_refs = [ref for ref in entry.get('uniProtKBCrossReferences', [])
            if ref['database'] == 'PDB']

Get Specific Fields (TSV)

def get_fields(query, fields):
    '''Get specific fields as DataFrame'''
    import pandas as pd
    from io import StringIO

    url = 'https://rest.uniprot.org/uniprotkb/search'
    params = {'query': query, 'format': 'tsv', 'fields': ','.join(fields), 'size': 500}
    response = requests.get(url, params=params)
    return pd.read_csv(StringIO(response.text), sep='\t')

df = get_fields('organism_id:9606 AND keyword:kinase AND reviewed:true',
                ['accession', 'gene_names', 'protein_name', 'length', 'go_p'])

Available Fields

accession

gene_names

protein_name

organism_name

length

mass

go_p

go_c

go_f

xref_pdb

ft_domain

ft_binding

Biopython Integration

from Bio import SeqIO
from io import StringIO

fasta_text = fetch_uniprot('P04637', 'fasta')
record = SeqIO.read(StringIO(fasta_text), 'fasta')
print(record.id, len(record.seq))

Related Skills

• database-access/entrez-fetch - NCBI protein access
• database-access/blast-searches - BLAST against UniProt
• structural-biology/structure-io - Download PDB structures
• structural-biology/alphafold-predictions - AlphaFold structures